» with protein or antibody using supplier catalogues or your own stock.

MicroBioChips recommends our selected partner Abcam for purchasing your favorite antibodies.
Transform your antibody / protein Research and Development into the age of reproducible proteomics.
Using our expertise and know-how to perform custom protein / peptide / antibody microarrays, you will be able to have reproducible results.
Example of application:
Serum proteins can be multiplexed for diagnostic / pronostic purposes in a R&D setting. In preliminary screening, these proteins can be identified by customers in 2D-gel, Maldi-Tof, Seldi-Tof, Lc-Ms or other proteomic or transcriptomic techniques. Then a custom antibody array is designed to capture these biomarkers in sera from patient groups, treated or non-treated, and then employed to validate the extent of biomarker significance. Ultimately, this array will provide companion diagnostic companies with a tool to predict a response to treatment. Format of the test could be ultimately become a multiplexed-ELISA based upon statistically significant results obtained from array-based population screening.

So, you can find below the results obtained on custom antibody / protein arrays, feel free to contact us for more information:

»  Example on custom multi-pad peptide array (100 peptides for epitope mapping) (example)

»  Example on custom antibody array (40 antibodies for diagnostic research) (example)

»  Example on custom antigen array (for vaccine research) (example)

Besides these examples, MicroBioChips develops a program in order to better characterize your antibodies as described below:



» Determination of dynamic range and sensitivity of Antibody / Antigen interaction.

Major value-add when dealing with very expensive / precious antigens, for use against a single antigen; x10 dilutions for both Ag and Ab; each in duplicate.



» A panel of up to 14 antibodies directed against antigens, up to 14 antigens.

For testing prior to use in co-location studies, FACS screening, cocktailing, etc.
Antibody panel tested against Antigen panel via 'odd-man-out' method (Antibody panel minus one Antigen at-a-time against static Antigen panel).


» Up to 400 spots per array.

Determined Antibody/ Antigen affinity by Surface Plasmon Resonance (Biacore technology) on multiplex technology; including determination of Ka and Kd.




Please visit our E-shop, in order to have more informations about our custom services on protein / antibody arrays.