The goal of an antibody array experiment is to compare the expression level of hundreds of proteins simultaneously between samples; to characterize pathways or to identify biomarkers.

It is possible to include around 100 diferent antibodies in duplicates on a 14-arrays slide. Around 500 in duplicates in a 2-arrays slide.

On one slide, 14 different samples can be analyzed, as a function of experiment size.

MicroBioChips can work with cell pellet, tissue, cell culture supernatant, serum,…

In order to realize the experiments in the better conditions,MicroBioChips needs at least: > 1 Million cells or 10mg of tissue, to extract and label >100µg of total protein extract; or 20 µl of serum.

Most manufacturers attempt to verify antigen recognition for their antibodies. MicroBioChips can check this specificity in the presence of unsurpassed antigenic diversity and deliver a much higher level of confidence that your antibodies will perform the task envisaged without the risk of wasting valuable time and energy in the laboratory.

In order to deliver the best results,MicroBioChips includes several controls: housekeeping proteins for normalization, labeled proteins for spotting, BSA for non-specific background.

The detection method depends on the type of experiment, MicroBioChips utilize 2 diferent methods : direct labeling (with Cy5) and indirect labeling (sandwich method). To obtain more information visit our section Principle

The microarray method is semi-quantitative. But it is possible on demand, to include a standard curve for real quantification.

MicroBioChips highly recommends to conduct experiments on 3 or more replicates.
If you wish to compare samples groups (e.g.: control versus test), it is recommended to have consistent biological samples and ideally group size able to facilitated meaningful statistical analysis.

For custom microarrays it is about 6 to 8 weeks, depending on the complexity of a tailored experiments